Submit a preprint


Multiproxy analysis exploring patterns of diet and disease in dental calculus and skeletal remains from a 19th century Dutch populationuse asterix (*) to get italics
Bartholdy, Bjørn Peare; Hasselstrøm, Jørgen B.; Sørensen, Lambert K.; Casna, Maia; Hoogland, Menno; Historisch Genootschap Beemster; Henry, Amanda G.Please use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"
<p>Dental calculus is an excellent source of information on the dietary patterns of past populations, including consumption of plant-based items. The detection of plant-derived residues such as alkaloids and their metabolites in dental calculus provides direct evidence of consumption by individuals within a population.&nbsp;</p> <p>We conducted a study on 41 individuals from Middenbeemster, a 19th century rural Dutch archaeological site. Skeletal and dental analysis was performed to explore potential relationships between pathological conditions/lesions and the presence of alkaloids. We also explored other factors potentially affecting the detection of alkaloids, including sample weight and skeletal preservation. Dental calculus was sampled and analysed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-ESI-MS/MS).</p> <p>We were able to detect nicotine, cotinine, caffeine, theophylline, and salicylic acid. By detecting these compounds we are able to show the consumption of tea and coffee and smoking of tobacco on an individual scale, which is also confirmed by historic documentation and identification of pipe notches in the dentition. Nicotine and/or cotinine was present in 56% of individuals with at least one visible pipe notch. We find some influence of skeletal preservation on the detection of alkaloids and salicylic acid, with higher quantities of compounds extracted from well-preserved individuals, and also observe a relationship between weight of the calculus sample and raw quantity of the detected compounds, and we were able to detect alkaloids in samples as small as 2 mg. We found correlations between chronic maxillary sinusitis and the presence of multiple alkaloids.</p> <p>We show that there are many limitations that will need to be addressed going forward with this type of analysis, and stress the need for more systematic research on the consumption of alkaloid-containing items and their subsequent concentration and preservation in dental calculus, in addition to how mode of consumption may affect concentrations on different parts of the dentition. Despite the limitations, this preliminary study illustrates the many benefits of using calculus to target a variety of compounds that could have been ingested as medicine or diet, or consumed in a different manner. This method allows us to directly address specific individuals, which can be especially useful in individuals that are not always well-documented in historic documentation, such as rural populations, and especially children and women.</p> should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https:// should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://
You should fill this box only if you chose 'Codes have been used in this study'. URL must start with http:// or https://
dental calculus; LC-MS/MS; alkaloids; dental pathology; sinusitis; caffeine; tobacco
NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).
Bioarchaeology, Post-medieval
Jelmer Eerkens [], Mario Zimmermann [], Shannon Tushingham [], Stephen Buckley [], Angelo Gismondi [] No need for them to be recommenders of PCIArchaeology. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct
e.g. John Doe []
2023-07-31 17:21:40
Louise Le Meillour
Mario Zimmerman, Anonymous