Tektites, a naturally occurring glass produced by major cosmic impacts and ejected at long distances, are known from five impacts worldwide [1]. The presence of this impact-generated glass, which can be dated in the same way as a volcanic rock, has been used to date archaeological sites in several regions of the world. This paper by Marwick and colleagues [2] reviews and adds new data on the use and misuse of this specific material as a chronological marker in Australia, East and Southeast Asia, where an impact dated to 0.78 Ma created and widely distributed tektites. This material, found in archaeological excavations in China, Laos, Thaïland, Australia, Borneo, and Vietnam, has been used to date layers containing lithic artifacts, sometimes creating a strong debate about the antiquity of the occupation and lithic production in certain regions.

The review of existing data shows that geomorphological data and stratigraphic integrity can be questioned at many sites that have yielded tektites. The new data provided by this paper for five archaeological sites located in Vietnam confirm that many deposits containing tektites are indeed lag deposits and that these artifacts, thus in secondary position, cannot be considered to date the layer. This study also emphasizes the general lack of other dating methods that would allow comparison with the tektite age. In the Vietnamese archaeological sites presented here, discrepancies between methods, and the presence of historical artifacts, confirm that the layers do not share similar age with the cosmic impact that created the tektites.

Based on this review and these new results, and following previous propositions [3], Marwick and colleagues conclude that, if tektites can be used as chronological markers, one has to prove that they are in situ. They propose that geomorphological assessment of the archaeological layer as primary deposit must first be attained, in addition to several parameters of the tektites themselves (shape, size distribution, chemical composition). Large error can be made by using only tektites to date an archaeological layer, and this material should not be used solely due to risks of high overestimation of the age of the archaeological production. 

[1] Rochette, P., Beck, P., Bizzarro, M., Braucher, R., Cornec, J., Debaille, V., Devouard, B., Gattacceca, J., Jourdan, F., Moustard, F., Moynier, F., Nomade, S., Reynard, B. (2021). Impact glasses from Belize represent tektites from the Pleistocene Pantasma impact crater in Nicaragua. Communications Earth & Environment, 2(1), 1–8, https://doi.org/10.1038/s43247-021-00155-1

[2] Marwick, B., Son, P. T., Brewer, R., Wang, L.-Y. (2022). Tektite geoarchaeology in mainland Southeast Asia. SocArXiv, 93fpa, ver. 6 peer-reviewed and recommended by PCI Archaeology, https://doi.org/10.31235/osf.io/93fpa.

[3] Tada, T., Tada, R., Chansom, P., Songtham, W., Carling, P. A., Tajika, E. (2020). In Situ Occurrence of Muong Nong-Type Australasian Tektite Fragments from the Quaternary Deposits near Huai Om, Northeastern Thailand. Progress in Earth and Planetary Science 7(1), 1–15, https://doi.org/10.1186/s40645-020-00378-4

Silica coating developed in cave art walls had an impact in the preservation of the paintings themselves. Despite it still exists a controversy about whether or not the effects contribute to the preservation of the artworks; it is evident that identifying these silica coatings would have an impact to assess the taphonomy of the walls and the paintings preserved on them. Unfortunately, current techniques -especially non-invasive ones- can hardly address amorphous silica characterisation. Thus, its presence is often detected on laboratory observations such as SEM or XRD analyses. In the paper “Light in the Cave: Opal coating detection by UV-light illumination and fluorescence in a rock art context - Methodological development and application in Points Cave (Gard, France)”, Quiers and collaborators propose a new method for the in situ detection and characterisation of amorphous silica in a rock art context based on UV laser-induced fluorescence (LIF) and UV illumination [1].

The results from both methods presented by the authors are convincing for the detection of U-silica mineralisation (U-opal in the specific case of study presented). This would allow access to a fast and cheap method to identify this kind of formations in situ in decorated caves. Beyond the relationship between opal coating and the preservation of the rock art, the detection of silica mineralisation can have further implications. First, it can help to define spot for sampling for pigment compositions, as well as reconstruct the chronology of the natural history of the caves and its relation with the human frequentation and activities. In conclusion, I am glad to recommend this original research, which offers a new approach to the identification of geological processes that affect -and can be linked with- the Palaeolithic cave art.

[1] Quiers, M., Chanteraud, C., Maris-Froelich, A., Chalmin-Aljanabi, E., Jaillet, S., Noûs, C., Pairis, S., Perrette, Y., Salomon, H., Monney, J. (2022) Light in the Cave: Opal coating detection by UV-light illumination and fluorescence in a rock art context. Methodological development and application in Points Cave (Gard, France). HAL, hal-03383193, ver. 5 peer-reviewed and recommended by Peer community in Archaeology. https://hal.archives-ouvertes.fr/hal-03383193v5

Establishing the precise location of ancient cities constitutes a challenging task that requires the implementation of multi-disciplinary approaches. In his manuscript entitled “Raphana of the Decapolis and its successor Arpha: The search of an eminent Greco-Roman city”, Kleb (2022) proposes a convincing argument building on in-depth research of classical literary sources, literature review of explorer accounts and scientific publications from the 19th and 20th century as well as analysis of old and new maps, aerial photographs, and satellite images. This research report clearly emphasizes the importance of undertaking systematic interdisciplinary work on the topic to mitigate the uncertainties associated with the identification of Raphana, the Decapolis city first mentioned by Pliny the Elder.

The Decapolis refers to a group of ten cities of Hellenistic traditions located on the eastern borders of the Roman Empire. This group of cities plays an important role in research that aims to contextualize the Judaean and Galilean history and to investigate urban centers in which different local and Greco-Roman influences met (Lichtenberger, 2021). While the location of most of the Decapolis cities is known and is (or was) subjected to systematic archaeological investigations (e.g., Eisenberg and Kowalewska, 2022; Makhadmeh et al., 2020; Shiyab et al., 2019), the location of others remain speculative. This is the case of Raphana for which the precise location remains difficult to establish owing in part to numerous name changes, limited information on the city structure, architecture, and size, etc.

The research presented by Kleb (2022) has some merits, which is emphasized here, although the report is presented in an unusual format compared to traditional scientific articles, i.e., introduction, research background, methodology, results, and discussion. First, the extensive review of classical works allows the reader to gain a historical perspective on the change of names from Raepta/Raphana to Arpha/Arefa. The author argues these different names likely refer to a single location. Second, the author combs through an impressive literature from the 19th and 20th century and emphasize how some assumptions by explorers who visited the region were introduced in the scientific literature and remained unchallenged. Finally, the author gathers a remarkable quantity of old and new maps of the Golan, el-Ledja and Hauran regions and compare them with multiple lines of evidence to hypothesize that the location of Raphana may lie near Ar-Rafi’ah, also known as Bir Qassab, in the Ard el Fanah plain, a conclusion that now requires to be tested through fieldwork investigations.

References

Kleb, J. (2022) Raphana of the Decapolis and its successor Arpha - The search for an eminent Greco-Roman City. Figshare, 20550021, ver. 4 peer-reviewed and recommended by Peer Community in Archaeology. https://doi.org/10.6084/m9.figshare.20550021

Eisenberg, M. and Kowalewska, A. (2022). Funerary podia of Hippos of the Decapolis and the phenomenon in the Roman world. J. Roman Archaeol. 35, 107–138. https://doi.org/10.1017/S1047759421000465

Lichtenberger, A. (2021). The Decapolis, in: A Companion to the Hellenistic and Roman Near East. John Wiley & Sons, Ltd, pp. 213–222. https://doi.org/10.1002/9781119037354.ch18

Makhadmeh, A., Al-Badarneh, M., Rawashdeh, A. and Al-Shorman, A. (2020). Evaluating the carrying capacity at the archaeological site of Jerash (Gerasa) using mathematical GIS modeling. Egypt. J. Remote Sens. Space Sci. 23, 159–165. https://doi.org/10.1016/j.ejrs.2018.09.002

Shiyab, A., Al-Shorman, A., Turshan, N., Tarboush, M., Alawneh, F. and Rahabneh, A. (2019). Investigation of late Roman pottery from Gadara of the Decapolis, Jordan using multi-methodic approach. J. Archaeol. Sci. Rep. 25, 100–115. https://doi.org/10.1016/j.jasrep.2019.04.003

Data authenticity and approaches to data authentication are crucial issues in ancient protein research. The advent of modern mass spectrometry has enabled the detection of traces of ancient biomolecules contained in fossils, including protein sequences. However, detecting proteins in ancient samples does not equate to demonstrating their endogenous nature: instead, if the mechanisms that drive protein preservation and degradation are understood, then the extent of protein diagenesis can be used for evaluating preservational quality, which in turn may be related to the authenticity of the protein data. 

The post-mortem deamidation of asparaginyl and glutamyl residues is a key degradation reaction, which can be assessed effectively on the basis of mass spectrometry data, and which has accrued a long history of research, both in terms of describing the mechanisms governing the reactions and with regard to the best strategies for assessing and quantifying the extent of glutamine (Gln) and asparagine (Asn) deamidation in ancient samples (Pal Chowdhury et al., 2019; Ramsøe et al., 2021, 2020; Schroeter and Cleland, 2016; Simpson et al., 2016; Solazzo et al., 2014; Welker et al., 2016; Wilson et al., 2012). 

In their paper, Nair and colleagues (2022) build on this wealth of knowledge and present a tool for quantifying the extent of Gln deamidation in parchment. Parchment is a collagen-based material which can yield extraordinary insights into manuscript manufacturing practices in the past, as well as on the daily lives of the people who assembled and used them (“biocodicology”) (Fiddyment et al., 2021, 2019, 2015; Teasdale et al., 2017). Importantly, the extent of deamidation can be directly related to the quality of the parchment produced: rapid direct deamidation of Gln is induced by the liming process, therefore high extents of deamidation are linked to prolonged exposure to the high pH conditions which are typical of liming, thus implying lower-quality parchment.

Nair et al.’s approach focuses on collagen peptides which are typically detected during MALDI-TOF mass spectrometry analyses of parchment and build a simple three-step workflow able to yield an overall index of deamidation for a sample (the parchment glutamine index - PQI) 一 taking into account that different Gln residues degrade at different rates according to their micro-chemical environment. The first step involves pre-processing the MALDI spectra, since Nair et al. are specifically interested in maximising information which can be obtained by low-quality data. The second step builds on well-established methods for quantifying Q → E from MALDI-TOF data by modelling the convoluted isotope distributions (Wilson et al., 2012). Once relative rates of deamidation in selected peptides within a given sample are calculated, the third step uses a mixed effects model to combine the individual deamidation estimates and to obtain an overall estimate of the deamidation for a parchment sample (PQI). 

The PQI can be used effectively for assessing parchment quality, as the authors show for the dataset from Orval Abbey. However, PQI could also have wider applications to the study of processed collagen, which is widely used in the food and pharmaceutical industries. In general, the study by Nair et al. is a welcome addition to a growing body of research on protein diagenesis, which will ultimately improve models for the assessment of the authenticity of biomolecular data in archaeology. 

References

Chowdhury, P.M., Wogelius, R., Manning, P.L., Metz, L., Slimak, L., and Buckley, M. 2019. Collagen deamidation in archaeological bone as an assessment for relative decay rates. Archaeometry 61:1382–1398. https://doi.org/10.1111/arcm.12492

Fiddyment, S., Goodison, N.J., Brenner, E., Signorello, S., Price, K., and Collins, M.J.. 2021. Girding the loins? Direct evidence of the use of a medieval parchment birthing girdle from biomolecular analysis. bioRxiv. https://doi.org/10.1098/rsos.202055

Fiddyment,S., Holsinger, B., Ruzzier, C., Devine, A., Binois, A., Albarella, U., Fischer, R., Nichols, E., Curtis, A., Cheese, E., Teasdale, M.D., Checkley-Scott, C., Milner, S.J., Rudy, K.M., Johnson, E.J., Vnouček, J., Garrison, M., McGrory, S., Bradley, D.G., and Collins, M.J. 2015. Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting. Proc Natl Acad Sci U S A 112:15066–15071. https://doi.org/10.1073/pnas.1512264112

Fiddyment, S., Teasdale, M.D., Vnouček, J., Lévêque, É., Binois, A., and Collins, M.J. 2019. So you want to do biocodicology? A field guide to the biological analysis of parchment. Heritage Science 7:35. https://doi.org/10.1186/s40494-019-0278-6

Nair, B., Rodríguez Palomo, I., Markussen, B., Wiuf, C., Fiddyment, S., and Collins, M. Parchment Glutamine Index (PQI): A novel method to estimate glutamine deamidation levels in parchment collagen obtained from low-quality MALDI-TOF data. BiorRxiv, 2022.03.13.483627, ver. 6 peer-reviewed and recommended by Peer community in Archaeology. https://doi.org/10.1101/2022.03.13.483627 

Ramsøe, A., Crispin, M., Mackie, M., McGrath, K., Fischer, R., Demarchi, B., Collins, M.J., Hendy, J., and Speller, C. 2021. Assessing the degradation of ancient milk proteins through site-specific deamidation patterns. Sci Rep 11:7795. https://doi.org/10.1038/s41598-021-87125-x

Ramsøe, A., van Heekeren, V., Ponce, P., Fischer, R., Barnes, I., Speller, C., and Collins, M.J. 2020. DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. J Archaeol Sci 115:105080. https://doi.org/10.1016/j.jas.2020.105080

Schroeter, E.R., and Cleland, T.P. 2016. Glutamine deamidation: an indicator of antiquity, or preservational quality? Rapid Commun Mass Spectrom 30:251–255. https://doi.org/10.1002/rcm.7445

Simpson, J.P., Penkman, K.E.H., and Demarchi, B. 2016. The effects of demineralisation and sampling point variability on the measurement of glutamine deamidation in type I collagen extracted from bone. J Archaeol Sci 69: 29-38. https://doi.org/10.1016/j.jas.2016.02.002

Solazzo, C., Wilson, J., Dyer, J.M., Clerens, S., Plowman, J.E., von Holstein, I., Walton Rogers, P., Peacock, E.E., and Collins, M.J. 2014. Modeling deamidation in sheep α-keratin peptides and application to archeological wool textiles. Anal Chem 86:567–575. https://doi.org/10.1021/ac4026362

Teasdale, M.D., Fiddyment, S., Vnouček, J., Mattiangeli, V., Speller, C., Binois, A., Carver, M., Dand, C., Newfield, T.P., Webb, C.C., Bradley, D.G., and Collins M.J. 2017. The York Gospels: a 1000-year biological palimpsest. R Soc Open Sci 4:170988. https://doi.org/10.1098/rsos.170988

Welker, F., Soressi, M.A., Roussel, M., van Riemsdijk, I., Hublin, J.-J., and Collins, M.J. 2016. Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen. STAR: Science & Technology of Archaeological Research 3:15–27. https://doi.org/10.1080/20548923.2016.1258825

Wilson, J., van Doorn, N.L., and Collins, M.J. 2012. Assessing the extent of bone degradation using glutamine deamidation in collagen. Anal Chem 84:9041–9048. https://doi.org/10.1021/ac301333t

The Levantine Corridor is considered a crossing point to Eurasia and one of the main areas for detecting population flows (and their associated cultural and economic changes) during the Pleistocene. This area could have been closed during the most arid periods, giving rise to processes of population isolation between Africa and Eurasia and intermittent contact between Eurasian human communities [1,2]. 

Zooarchaeological studies of the early Upper Palaeolithic assemblages constitute an important source of knowledge about human subsistence, making them central to the debate on modern behaviour. The Early Upper Palaeolithic sequence in the Levant includes two cultural entities – the Early Ahmarian and the Levantine Aurignacian. This latter is dated to 39-33 ka and is considered a local adaptation of the European Aurignacian techno-complex. In this work, the authors present a zooarchaeological study of the Nahal Rahaf 2 (ca. 35 ka) archaeological site in the southern Judean Desert in Israel [3].

Zooarchaeological data from the early Upper Paleolithic desert regions of the southern Levant are not common due to preservation problems of non-lithic finds. In the case of Nahal Rahaf 2, recent excavation seasons brought to light a stratigraphical sequence composed of very well-preserved archaeological surfaces attributed to the 'Arkov-Divshon' cultural entity, which is associated with the Levantine Aurignacian. 

This study shows age-specific caprine (Capra cf. Capra ibex) hunting on prime adults and a generalized procurement of gazelles (Gazella cf. Gazella gazella), which seem to have been selectively transported to the site and processed for within-bone nutrients. An interesting point to note is that the proportion of goats increases along the stratigraphic sequence, which suggests to the authors a specialization in the economy over time that is inversely related to the occupational intensity of use of the site. 

It is also noteworthy that the materials represent a large sample compared to previous studies from the Upper Paleolithic of the Judean Desert and Negev.

In summary, this manuscript contributes significantly to the study of both the palaeoenvironment and human subsistence strategies in the Upper Palaeolithic and provides another important reference point for evaluating human hunting adaptations in the arid regions of the southern Levant.

References

[1] Bermúdez de Castro, J.-L., Martinon-Torres, M. (2013). A new model for the evolution of the human pleistocene populations of Europe. Quaternary Int. 295, 102-112. https://doi.org/10.1016/j.quaint.2012.02.036

[2] Bar-Yosef, O., Belfer-Cohen, A. (2010). The Levantine Upper Palaeolithic and Epipalaeolithic. In Garcea, E.A.A. (Ed), South-Eastern Mediterranean Peoples Between 130,000 and 10,000 Years Ago. Oxbow Books, pp. 144-167.

[3] Marom, N., Gnezdilov, D. L., Shafir, R., Barzilai, O. and Shemer, M. (2022). Faunal remains from the Upper Paleolithic site of Nahal Rahaf 2 in the southern Judean Desert, Israel. BioRxiv, 2022.05.17.492258, ver. 4 peer-reviewed and recommended by Peer community in Archaeology. https://www.biorxiv.org/content/10.1101/2022.05.17.492258v4