The Levantine Corridor is considered a crossing point to Eurasia and one of the main areas for detecting population flows (and their associated cultural and economic changes) during the Pleistocene. This area could have been closed during the most arid periods, giving rise to processes of population isolation between Africa and Eurasia and intermittent contact between Eurasian human communities [1,2]. 

Zooarchaeological studies of the early Upper Palaeolithic assemblages constitute an important source of knowledge about human subsistence, making them central to the debate on modern behaviour. The Early Upper Palaeolithic sequence in the Levant includes two cultural entities – the Early Ahmarian and the Levantine Aurignacian. This latter is dated to 39-33 ka and is considered a local adaptation of the European Aurignacian techno-complex. In this work, the authors present a zooarchaeological study of the Nahal Rahaf 2 (ca. 35 ka) archaeological site in the southern Judean Desert in Israel [3].

Zooarchaeological data from the early Upper Paleolithic desert regions of the southern Levant are not common due to preservation problems of non-lithic finds. In the case of Nahal Rahaf 2, recent excavation seasons brought to light a stratigraphical sequence composed of very well-preserved archaeological surfaces attributed to the 'Arkov-Divshon' cultural entity, which is associated with the Levantine Aurignacian. 

This study shows age-specific caprine (Capra cf. Capra ibex) hunting on prime adults and a generalized procurement of gazelles (Gazella cf. Gazella gazella), which seem to have been selectively transported to the site and processed for within-bone nutrients. An interesting point to note is that the proportion of goats increases along the stratigraphic sequence, which suggests to the authors a specialization in the economy over time that is inversely related to the occupational intensity of use of the site. 

It is also noteworthy that the materials represent a large sample compared to previous studies from the Upper Paleolithic of the Judean Desert and Negev.

In summary, this manuscript contributes significantly to the study of both the palaeoenvironment and human subsistence strategies in the Upper Palaeolithic and provides another important reference point for evaluating human hunting adaptations in the arid regions of the southern Levant.

References

[1] Bermúdez de Castro, J.-L., Martinon-Torres, M. (2013). A new model for the evolution of the human pleistocene populations of Europe. Quaternary Int. 295, 102-112. https://doi.org/10.1016/j.quaint.2012.02.036

[2] Bar-Yosef, O., Belfer-Cohen, A. (2010). The Levantine Upper Palaeolithic and Epipalaeolithic. In Garcea, E.A.A. (Ed), South-Eastern Mediterranean Peoples Between 130,000 and 10,000 Years Ago. Oxbow Books, pp. 144-167.

[3] Marom, N., Gnezdilov, D. L., Shafir, R., Barzilai, O. and Shemer, M. (2022). Faunal remains from the Upper Paleolithic site of Nahal Rahaf 2 in the southern Judean Desert, Israel. BioRxiv, 2022.05.17.492258, ver. 4 peer-reviewed and recommended by Peer community in Archaeology. https://www.biorxiv.org/content/10.1101/2022.05.17.492258v4

Bischoff (2023) is a significant contribution to the growing field of geometric morphometric analysis in stone tool analysis. The subject is projectile points from the southwestern United States. Projectile point typologies or systematics remain an important part of North American archaeology, and in fact these typologies continue to be used primarily as cultural-historical markers. This article looks at projectile point types using a 2D image geometric morphometric analysis as a way of both improving on projectile point types but also testing if these types are in fact based in measurable reality. A total of 164 point outlines are analyzed using Elliptical Fourier, semilandmark and landmark analyses. The author also uses a network analysis to look at possible relationships between projectile point morphologies in space. This is a clever way of working around the predefined distributions of projectile point types, some of which are over 100 years old. Because of the dynamic nature of stone tools in terms of their use, reworking and reuse, this article can also provide solutions for studying the dynamic nature of stone tools. This article therefore also has a wide applicability to other stone tool analyses.

Reference

Bischoff, R. J. (2023) Geometric Morphometric Analysis of Projectile Points from the Southwest United States, SocArXiv, a6wjc, ver. 8 peer-reviewed and recommended by Peer Community in Archaeology. https://doi.org/10.31235/osf.io/a6wjc

The paper entitled “Investigating relationships between technological variability and ecology in the Middle Gravettian (ca. 32-28 ky cal. BP) in France” [1] submitted by A. Vignoles and colleagues offers a robust and interesting new analysis of the niche differences between the Rayssian and Noaillian facies of the Middle Gravettian in France.

Understanding technological variability in the Palaeolithic is a long-standing challenge. Previous debates have vacillated between strong, quasi-ethnic culture-historical interpretations rooted in the traditional European school and extreme functional stances that would see artefact forms and their frequencies with assemblages conditioned by site function. While both positions have their merits, many empirical and conceptual caveats haunt them equally [see 2]. In this new study Vignoles and colleagues, so-called eco-cultural niche modelling is applied in an attempt to explore whether, and if so, which environmental background factors may have conditioned the emergence and persistence of two sub-cultural categories (facies) within the Middle Gravettian: the Rayssian and the Noaillian. These are are defined through, respectively, a specific knapping method and the presence of a specific burin type, and the occurrence of these seems divided by the Garonne River. Eco-cultural niche modelling has emerged as an archaeological application of distribution models widely employed in ecology, including palaeoecology, to understand organismal niche envelopes [3]. They constitute powerful tools for using the spatial and chronological information inherent in the archaeological record to up-scale interpretations of human-environment relations beyond individual site stratigraphies or dating series. Another important feature of such models is that their performance can, as Vignoles et al. also show, be formally evaluated and replicated. Following on from earlier applications of such techniques [e.g. 4], the authors here present an interesting study that uses very specific archaeological indicators – namely the Raysse method and the Noaillian burin – as defining features for the units (communities, traditions) whose adaptations they investigate. While broad tool types have previously been used as cultural taxonomic indicators in niche modelling studies [5], the present study is ambitious in its attempt to understand variability at a relatively small spatial scale. This mirrors equally interesting attempts of doing so in later prehistoric contexts [6].

Applications of niche modelling that use analytical units defined through archaeological characteristics (technology, typology) are opening up exciting new opportunities for pinning down precisely which environmental or climatic features these cultural components reference, if any. The study by Vignoles et al. makes a good case. At the same time, this approach also acutely raises questions of cultural taxonomy, of how we define our units of analysis and what they might mean [7]. It remains unclear to whether we can define such units on the basis of very different technological traits if the aim is to then use them as taxonomically equivalent in subsequent analyses. There is also a risk that these facies become reified as traditions of sub-cultures – then often further equated with specific people – through an overly normative view of their constituent technological elements. In addition, studies of adaptation in principle need to be conscious of the so-called ‘Galton’s Problem’, where the historical relatedness of the analytical units in question need to be taken into account in seeking salient correlations between cultural and environmental features [8]. In pushing forward eco-cultural niche modelling, the study by Vignoles et al. thus takes us some way forward in understanding the potentially adaptive variability within the Gravettian; future work should consider more strongly the specific historical relatedness amongst the cultural taxa under study and follow more theory-driven definition thereof. Such definition would also allow the post-analysis interpretations of eco-cultural niche modelling to be more explicit. Without doubt, the Gravettian as a whole – including, for instance, phenomena such as the Maisierian [9] – would benefit from additional and extended applications of this method. Similarly, other periods of the Palaeolithic also characterized by such variability (e.g. the Magdalenian and Final Palaeolithic) offer additional cases moving forward.

Bibliography

[1] Vignoles, A. et al. (2020). Investigating relationships between technological variability and ecology in 1 the Middle Gravettian (ca. 32-28 ky cal. BP) in France. PCI Archaeology. 10.31219/osf.io/ud3hj

[2] Dibble, H.L., Holdaway, S.J., Lin, S.C., Braun, D.R., Douglass, M.J., Iovita, R., McPherron, S.P., Olszewski, D.I., Sandgathe, D., 2017. Major Fallacies Surrounding Stone Artifacts and Assemblages. Journal of Archaeological Method and Theory 24, 813–851. 10.1007/s10816-016-9297-8

[3] Svenning, J.-C., Fløjgaard, C., Marske, K.A., Nógues-Bravo, D., Normand, S., 2011. Applications of species distribution modeling to paleobiology. Quaternary Science Reviews 30, 2930–2947. 10.1016/j.quascirev.2011.06.012

[4] Banks, W.E., d’Errico, F., Dibble, H.L., Krishtalka, L., West, D., Olszewski, D.I., Townsend Petersen, A., Anderson, D.G., Gillam, J.C., Montet-White, A., Crucifix, M., Marean, C.W., Sánchez-Goñi, M.F., Wolfarth, B., Vanhaeren, M., 2006. Eco-Cultural Niche Modeling: New Tools for Reconstructing the Geography and Ecology of Past Human Populations. PaleoAnthropology 2006, 68–83.

[5] Banks, W.E., Zilhão, J., d’Errico, F., Kageyama, M., Sima, A., Ronchitelli, A., 2009. Investigating links between ecology and bifacial tool types in Western Europe during the Last Glacial Maximum. Journal of Archaeological Science 36, 2853–2867. 10.1016/j.jas.2009.09.014

[6] Whitford, B.R., 2019. Characterizing the cultural evolutionary process from eco-cultural niche models: niche construction during the Neolithic of the Struma River Valley (c. 6200–4900 BC). Archaeological and Anthropological Sciences 11, 2181–2200. 10.1007/s12520-018-0667-x

[7] Reynolds, N., Riede, F., 2019. House of cards: cultural taxonomy and the study of the European Upper Palaeolithic. Antiquity 93, 1350–1358. 10.15184/aqy.2019.49

[8] Mace, R., Pagel, M.D., 1994. The Comparative Method in Anthropology. Current Anthropology 35, 549–564. 10.1086/204317

[9] Pesesse, D., 2017. Is it still appropriate to talk about the Gravettian? Data from lithic industries in Western Europe. Quartär 64, 107–128. 10.7485/QU64_5

Data authenticity and approaches to data authentication are crucial issues in ancient protein research. The advent of modern mass spectrometry has enabled the detection of traces of ancient biomolecules contained in fossils, including protein sequences. However, detecting proteins in ancient samples does not equate to demonstrating their endogenous nature: instead, if the mechanisms that drive protein preservation and degradation are understood, then the extent of protein diagenesis can be used for evaluating preservational quality, which in turn may be related to the authenticity of the protein data. 

The post-mortem deamidation of asparaginyl and glutamyl residues is a key degradation reaction, which can be assessed effectively on the basis of mass spectrometry data, and which has accrued a long history of research, both in terms of describing the mechanisms governing the reactions and with regard to the best strategies for assessing and quantifying the extent of glutamine (Gln) and asparagine (Asn) deamidation in ancient samples (Pal Chowdhury et al., 2019; Ramsøe et al., 2021, 2020; Schroeter and Cleland, 2016; Simpson et al., 2016; Solazzo et al., 2014; Welker et al., 2016; Wilson et al., 2012). 

In their paper, Nair and colleagues (2022) build on this wealth of knowledge and present a tool for quantifying the extent of Gln deamidation in parchment. Parchment is a collagen-based material which can yield extraordinary insights into manuscript manufacturing practices in the past, as well as on the daily lives of the people who assembled and used them (“biocodicology”) (Fiddyment et al., 2021, 2019, 2015; Teasdale et al., 2017). Importantly, the extent of deamidation can be directly related to the quality of the parchment produced: rapid direct deamidation of Gln is induced by the liming process, therefore high extents of deamidation are linked to prolonged exposure to the high pH conditions which are typical of liming, thus implying lower-quality parchment.

Nair et al.’s approach focuses on collagen peptides which are typically detected during MALDI-TOF mass spectrometry analyses of parchment and build a simple three-step workflow able to yield an overall index of deamidation for a sample (the parchment glutamine index - PQI) 一 taking into account that different Gln residues degrade at different rates according to their micro-chemical environment. The first step involves pre-processing the MALDI spectra, since Nair et al. are specifically interested in maximising information which can be obtained by low-quality data. The second step builds on well-established methods for quantifying Q → E from MALDI-TOF data by modelling the convoluted isotope distributions (Wilson et al., 2012). Once relative rates of deamidation in selected peptides within a given sample are calculated, the third step uses a mixed effects model to combine the individual deamidation estimates and to obtain an overall estimate of the deamidation for a parchment sample (PQI). 

The PQI can be used effectively for assessing parchment quality, as the authors show for the dataset from Orval Abbey. However, PQI could also have wider applications to the study of processed collagen, which is widely used in the food and pharmaceutical industries. In general, the study by Nair et al. is a welcome addition to a growing body of research on protein diagenesis, which will ultimately improve models for the assessment of the authenticity of biomolecular data in archaeology. 

References

Chowdhury, P.M., Wogelius, R., Manning, P.L., Metz, L., Slimak, L., and Buckley, M. 2019. Collagen deamidation in archaeological bone as an assessment for relative decay rates. Archaeometry 61:1382–1398. https://doi.org/10.1111/arcm.12492

Fiddyment, S., Goodison, N.J., Brenner, E., Signorello, S., Price, K., and Collins, M.J.. 2021. Girding the loins? Direct evidence of the use of a medieval parchment birthing girdle from biomolecular analysis. bioRxiv. https://doi.org/10.1098/rsos.202055

Fiddyment,S., Holsinger, B., Ruzzier, C., Devine, A., Binois, A., Albarella, U., Fischer, R., Nichols, E., Curtis, A., Cheese, E., Teasdale, M.D., Checkley-Scott, C., Milner, S.J., Rudy, K.M., Johnson, E.J., Vnouček, J., Garrison, M., McGrory, S., Bradley, D.G., and Collins, M.J. 2015. Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting. Proc Natl Acad Sci U S A 112:15066–15071. https://doi.org/10.1073/pnas.1512264112

Fiddyment, S., Teasdale, M.D., Vnouček, J., Lévêque, É., Binois, A., and Collins, M.J. 2019. So you want to do biocodicology? A field guide to the biological analysis of parchment. Heritage Science 7:35. https://doi.org/10.1186/s40494-019-0278-6

Nair, B., Rodríguez Palomo, I., Markussen, B., Wiuf, C., Fiddyment, S., and Collins, M. Parchment Glutamine Index (PQI): A novel method to estimate glutamine deamidation levels in parchment collagen obtained from low-quality MALDI-TOF data. BiorRxiv, 2022.03.13.483627, ver. 6 peer-reviewed and recommended by Peer community in Archaeology. https://doi.org/10.1101/2022.03.13.483627 

Ramsøe, A., Crispin, M., Mackie, M., McGrath, K., Fischer, R., Demarchi, B., Collins, M.J., Hendy, J., and Speller, C. 2021. Assessing the degradation of ancient milk proteins through site-specific deamidation patterns. Sci Rep 11:7795. https://doi.org/10.1038/s41598-021-87125-x

Ramsøe, A., van Heekeren, V., Ponce, P., Fischer, R., Barnes, I., Speller, C., and Collins, M.J. 2020. DeamiDATE 1.0: Site-specific deamidation as a tool to assess authenticity of members of ancient proteomes. J Archaeol Sci 115:105080. https://doi.org/10.1016/j.jas.2020.105080

Schroeter, E.R., and Cleland, T.P. 2016. Glutamine deamidation: an indicator of antiquity, or preservational quality? Rapid Commun Mass Spectrom 30:251–255. https://doi.org/10.1002/rcm.7445

Simpson, J.P., Penkman, K.E.H., and Demarchi, B. 2016. The effects of demineralisation and sampling point variability on the measurement of glutamine deamidation in type I collagen extracted from bone. J Archaeol Sci 69: 29-38. https://doi.org/10.1016/j.jas.2016.02.002

Solazzo, C., Wilson, J., Dyer, J.M., Clerens, S., Plowman, J.E., von Holstein, I., Walton Rogers, P., Peacock, E.E., and Collins, M.J. 2014. Modeling deamidation in sheep α-keratin peptides and application to archeological wool textiles. Anal Chem 86:567–575. https://doi.org/10.1021/ac4026362

Teasdale, M.D., Fiddyment, S., Vnouček, J., Mattiangeli, V., Speller, C., Binois, A., Carver, M., Dand, C., Newfield, T.P., Webb, C.C., Bradley, D.G., and Collins M.J. 2017. The York Gospels: a 1000-year biological palimpsest. R Soc Open Sci 4:170988. https://doi.org/10.1098/rsos.170988

Welker, F., Soressi, M.A., Roussel, M., van Riemsdijk, I., Hublin, J.-J., and Collins, M.J. 2016. Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen. STAR: Science & Technology of Archaeological Research 3:15–27. https://doi.org/10.1080/20548923.2016.1258825

Wilson, J., van Doorn, N.L., and Collins, M.J. 2012. Assessing the extent of bone degradation using glutamine deamidation in collagen. Anal Chem 84:9041–9048. https://doi.org/10.1021/ac301333t

The paper entitled “Probabilistic Modelling using Monte Carlo Simulation for Incorporating Uncertainty in Least Cost Path Results: a Roman Road Case Study” [1] submitted by J. Lewis presents an innovative approach to applying Least Cost Path (LCP) analysis to incorporate uncertainty of the Digital Elevation Model used as the topographic surface on which the path is calculated.

The proposition of using Monte Carlo simulations to produce numerous LCP, each with a slightly different DEM included in the error range of the model, allows one to strengthen the method by proposing a probabilistic LCP rather than a single and arbitrary one which does not take into account the uncertainty of the topographic reconstruction. This new method is integrated in the R package leastcostpath [2].

The author tests the method using a Roman road built along a ridge in Cumbria, England. The integration of the uncertainty of the DEM, thanks to Monte Carlo simulations, shows that two paths could have the same probability to be the real LCP. One of them is indeed the path that the Roman road took. In particular, it is one of two possibilities of LCP in the south to north direction.

This new probabilistic method therefore strengthens the reconstruction of past pathways, while also allowing new hypotheses to be tested, and, in this case study, to suggest that the northern part of the Roman road’s location was selected to help the northward movements.

[1] Lewis, J., 2020. Probabilistic Modelling using Monte Carlo Simulation for Incorporating Uncertainty in Least Cost Path Results: a Roman Road Case Study. SocArXiv, mxas2, ver 17 peer-reviewed and recommended by PCI Archaeology, 10.31235/osf.io/mxas2.

[2] Lewis, J., 2020. leastcostpath: Modelling Pathways and Movement Potential Within a Landscape. R package. Version 1.7.4.